GAP-43 gene expression regulates information storage
نویسندگان
چکیده
منابع مشابه
Factors influencing GAP-43 gene expression in PC12 pheochromocytoma cells.
We have studied factors controlling message levels for the neuronal growth- and plasticity-associated protein, GAP-43. Following exposure of PC12 cells to various effectors, cytoplasmic RNA was isolated and analyzed by Northern transfer and autoradiography using a GAP-43 cDNA probe. Induction by NGF is apparent after 3 hr exposure and reaches maximal levels at 24 hr. Beyond 24 hr, levels remain...
متن کاملThe RNA-binding protein HuD is required for GAP-43 mRNA stability, GAP-43 gene expression, and PKC-dependent neurite outgrowth in PC12 cells.
The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs. pDuH-transfected cells contained reduced amounts of GAP-43 protein and mRNA, ...
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Using transgenic mice, we have examined the expression pattern conferred by regions of genomic GAP-43 coupled to beta-galactosidase. We demonstrate that gene constructions that include the GAP-43 5'-flanking region along with sufficient sequences of the first intron drive beta-galactosidase (lacZ) expression to mimic in many regards the complex spatial and temporal pattern of endogenous GAP-43 ...
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Cadmium (Cd) is a phytotoxic heavy metal (HM) that can induce generation of reactive oxygen species (ROS). Arbuscular mycorrhizal fungi (AMF) are considered as bio-ameliorators that help to mitigate HM-derived oxidative stress. The objective of this study was to assess AM fungus Rhizophagus irregularis on changes in enzymatic activity and transcription of antioxidants of Medicago sativa to Cd s...
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Objective(s): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression. In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. Materials and Methods:Expression of CD11b and CD...
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ژورنال
عنوان ژورنال: Learning & Memory
سال: 2007
ISSN: 1072-0502
DOI: 10.1101/lm.581907